Research Associate CV — page 1

Dr

.

Elena

Vasquez

Research

Associate

·

Molecular

Biology

elena

.

vasquez

@

researchlab

.

org

+1 (415) 555-0192

San

Francisco

,

CA

linkedin

.

com

/

in

/

elenavasquez

github

.

com

/

e

-

venasquez

PROFESSIONAL

SUMMARY

Research

Associate

with

5+

years

of

experience

in

molecular

biology

,

genomic

analysis

,

and

cell

-

based

assays

.

Skilled

in

CRISPR

-

Cas

9

gene

editing

,

high

-

throughput

screening

,

and

bioinformatics

pipeline

development

.

Proven

track

record

of

contributing

to

peer

-

reviewed

publications

and

managing

cross

-

functional

research

projects

in

academic

and

biotech

settings

.

Passionate

about

translating

fundamental

biological

discoveries

into

therapeutic

applications

.

RESEARCH

EXPERIENCE

Research

Associate

Sep

2022 –

Present

Genentech

Inc

. ·

South

San

Francisco

,

CA

Research

Associate

Jan

2020 –

Aug

2022

Stanford

University

School

of

Medicine

·

Stanford

,

CA

Lead

a

team

of

3

research

assistants

investigating

epigenetic

regulation

in

oncology

targets

using

CRISPR

-

dCas

9

fusion

systems

.

Designed

and

executed

pooled

CRISPR

screens

(

genome

-

scale

and

targeted

)

identifying

14

novel

synthetic

lethal

interactions

in

triple

-

negative

breast

cancer

cell

lines

.

Developed

an

automated

high

-

content

imaging

pipeline

reducing

data

acquisition

time

by

40%

while

maintaining

>95%

assay

reproducibility

.

Collaborated

with

computational

biology

group

to

integrate

single

-

cell

RNA

-

seq

data

with

CRISPR

screen

results

,

uncovering

two

previously

unknown

resistance

mechanisms

.

Presented

findings

at

3

international

conferences

including

AACR

Annual

Meeting

2024

and

Keystone

Symposia

.

Managed

daily

operations

of

a

molecular

biology

lab

studying

RNA

-

binding

protein

dysregulation

in

neurodegeneration

.

Executed

RNA

-

seq

,

RIP

-

seq

,

and

CLIP

-

seq

library

preparation

protocols

;

analyzed

differential

expression

and

splicing

events

using

STAR

,

DESeq

2,

and

rMATS

.

Optimized

lentiviral

production

and

transduction

protocols

achieving

titers

consistently

above

1×10

⁸

TU

/

mL

for

primary

neuron

and

iPSC

-

derived

models

.

Trained

and

mentored

6

graduate

students

and

4

undergraduate

researchers

in

molecular

cloning

,

cell

culture

,

and

data

analysis

best

practices

.

Co

-

authored

3

manuscripts

in

high

-

impact

journals

(

Nature

Neuroscience

,

Molecular

Cell

)

with

one

first

-

author

publication

.

1 / 3

Research Associate CV — page 2

Junior

Research

Scientist

Jun

2017 –

Dec

2019

Broad

Institute

of

MIT

and

Harvard

·

Cambridge

,

MA

EDUCAT ION

Ph

.

D

.

in

Molecular

and

Cell

Biology

2012 – 2017

University

of

California

,

Berkeley

GPA

: 3.92

Dissertation

: “

Mechanisms

of

RNA

localization

in

neuronal

development

and

plasticity

.”

Advisor

:

Dr

.

Jennifer

A

.

Doudna

.

NIH

NRSA

Predoctoral

Fellow

.

B

.

S

.

in

Biochemistry

and

Molecular

Biology

2008 – 2012

University

of

Michigan

,

Ann

Arbor

GPA

: 3.88 ·

Magna

Cum

Laude

Honors

thesis

: “

Characterization

of

SUMOylation

dynamics

in

DNA

damage

repair

pathways

.”

Undergraduate

Research

Opportunity

Program

grant

recipient

.

T ECHNICAL

SKILLS

Molecular

CRISPR

-

Cas

9 (

pooled

,

arrayed

,

prime

editing

),

qPCR

/

ddPCR

,

cloning

,

Gibson

assembly

,

Western

blot

,

Co

-

IP

,

RIP

-

seq

,

CLIP

-

seq

Cell

Biology

Mammalian

cell

culture

(

HEK

293

T

,

HeLa

,

K

562,

iPSC

-

derived

neurons

),

lentivirus

/

AAV

production

,

flow

cytometry

,

high

-

content

imaging

(

Opera

Phenix

)

Genomics

RNA

-

seq

,

single

-

cell

RNA

-

seq

(10

x

Genomics

),

ATAC

-

seq

,

ChIP

-

seq

,

whole

-

exome

sequencing

,

CRISPR

amplicon

sequencing

Bioinformatics

Python

(

pandas

,

NumPy

,

Scanpy

),

R

(

DESeq

2,

Seurat

,

ggplot

2),

Snakemake

,

bash

,

Git

,

Docker

,

SLURM

HPC

,

AWS

cloud

computing

Assays

Cell

viability

(

CellTiter

-

Glo

,

MTT

),

apoptosis

(

Annexin

-

V

,

caspase

activity

),

proliferation

(

EdU

,

CFSE

),

ELISA

,

AlphaLISA

,

IncuCyte

live

-

cell

analysis

Other

ELN

(

Benchling

,

LabArchives

),

GraphPad

Prism

,

ImageJ

/

Fiji

,

FlowJo

,

Adobe

Illustrator

,

project

management

(

Asana

,

Trello

)

SELECT ED

PUBLICAT IONS

Vasquez

E

.

,

Park

J

.,

Liu

A

.

H

.,

et

al

. (2024).

Epigenetic

rewiring

drives

resistance

to

CDK

4/6

inhibitors

in

triple

-

negative

breast

cancer

.

Nature

Cell

Biology

,

26(4), 612–

625.

Nguyen

T

.

Q

.,

Vasquez

E

.

,

Soriano

A

.,

et

al

. (2023).

A

genome

-

wide

CRISPR

screen

identifies

novel

regulators

of

tau

aggregation

in

iPSC

-

derived

neurons

.

Neuron

,

111(8), 1201–1218.

Vasquez

E

.

,

Chen

Y

.,

O

'

Malley

M

. (2022).

RNA

-

binding

protein

FMRP

controls

local

translation

of

synaptic

plasticity

genes

in

hippocampal

neurons

.

Molecular

Cell

,

82(3), 543–560.

Schmidt

K

.,

Vasquez

E

.

,

Doudna

J

.

A

. (2021).

Kinetic

determinants

of

CRISPR

-

Cas

9

off

-

target

activity

in

human

cells

.

Nature

Communications

,

12, 3154.

HONORS

&

AWARDS

Contributed

to

a

large

-

scale

functional

genomics

project

characterizing

essential

genes

across

350+

cancer

cell

lines

using

CRISPR

-

Cas

9

dropout

screens

.

Developed

and

validated

a

multiplexed

qPCR

-

based

genotyping

panel

for

isogenic

cell

line

authentication

,

achieving

99.8%

concordance

with

WGS

data

.

Processed

and

analyzed

NGS

data

(

whole

-

exome

,

targeted

amplicon

,

CRISPR

amplicon

)

using

a

custom

Snakemake

pipeline

deployed

on

an

HPC

cluster

.

Maintained

laboratory

inventory

,

equipment

calibration

schedules

,

and

biosafety

compliance

documentation

across

3

shared

lab

spaces

.

NIH

Ruth

L

.

Kirschstein

NRSA

Predoctoral

Fellowship

(

F

31) · 2015–2017

Keystone

Symposia

Future

of

Science

Scholarship

· 2024

Genentech

Postdoctoral

Publication

Award

·

2023

UC

Berkeley

Graduate

Research

Fellowship

·

2013–2017

Phi

Beta

Kappa

·

University

of

Michigan

· 2012

Goldwater

Scholar

Honorable

Mention

· 2011

2 / 3

Research Associate CV — page 3

Vasquez

E

.

,

Thompson

L

. (2020).

Functional

characterization

of

RNA

localization

elements

in

the

3′

UTR

of

CAMK

2

A

.

RNA

,

26(9), 1190–1205.

PROFESSIONAL

ACT IVIT IES

&

CERT IFICAT IONS

Certifications

:

CITI

Program

Human

Research

(

Biomedical

) ·

IACUC

Animal

Handler

Training

·

BSL

-2

and

BSL

-2+

Biosafety

Certified

·

R

Programming

for

Data

Science

(

Johns

Hopkins

/

Coursera

).

Ad

-

hoc

Reviewer

:

Nucleic

Acids

Research

,

Cell

Reports

,

Scientific

Reports

,

PLOS

ONE

.

Professional

Memberships

:

American

Association

for

Cancer

Research

(

AACR

),

American

Society

for

Cell

Biology

(

ASCB

),

RNA

Society

.

Languages

:

English

(

native

),

Spanish

(

fluent

),

French

(

professional

proficiency

).

3 / 3

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