Laboratory
Testing
—
Hard
Skills
01
Specimen
Collection
&
Handling
02
Clinical
Chemistry
&
Immunoassay
03
Hematology
&
Coagulation
04
Microbiology
&
Culture
05
Molecular
Diagnostics
06
Immunology
&
Serology
Venipuncture
and
capillary
(
fingerstick
)
collection
✓
Phlebotomy
order
of
draw
and
tube
additive
selection
✓
Blood
culture
collection
using
aseptic
technique
✓
Urine
collection
:
clean
-
catch
,
midstream
, 24-
hour
✓
Swab
collection
:
nasopharyngeal
,
wound
,
MRSA
screening
✓
Specimen
labeling
,
accessioning
,
and
chain
of
custody
✓
Specimen
processing
:
centrifugation
,
aliquoting
,
serum
/
plasma
separation
✓
Transport
temperature
,
stability
,
and
light
-
protection
requirements
✓
Rejection
criteria
and
specimen
integrity
assessment
✓
Automated
chemistry
analyzers
(
Roche
Cobas
,
Abbott
Architect
,
Siemens
Atellica
)
✓
Spectrophotometry
and
endpoint
versus
kinetic
assays
✓
Electrolyte
panels
and
blood
gas
analysis
✓
Enzyme
-
linked
immunosorbent
assay
(
ELISA
)
✓
Chemiluminescent
immunoassay
(
CLIA
)
✓
Therapeutic
drug
monitoring
✓
Tumor
marker
and
hormone
assays
✓
Calibration
curve
generation
and
linearity
verification
✓
Point
-
of
-
care
testing
(
POCT
)
operation
and
quality
control
✓
Automated
CBC
analysis
(
Sysmex
,
Beckman
Coulter
)
✓
Manual
differential
and
peripheral
blood
smear
morphology
✓
Reticulocyte
counts
and
corrected
indices
✓
ESR
and
body
fluid
cell
counts
✓
PT
/
INR
,
aPTT
,
fibrinogen
,
and
D
-
dimer
testing
✓
Coagulation
mixing
studies
and
factor
assays
✓
Platelet
aggregation
and
von
Willebrand
factor
testing
✓
Sickle
cell
screening
and
hemoglobin
electrophoresis
✓
Bone
marrow
preparation
and
special
stains
✓
Aseptic
technique
and
biological
safety
cabinet
(
BSC
)
operation
✓
Streak
plate
isolation
and
colony
morphology
interpretation
✓
Gram
stain
and
acid
-
fast
staining
✓
Blood
,
urine
,
wound
,
and
respiratory
culture
workup
✓
MALDI
-
TOF
and
VITEK
organism
identification
✓
Antimicrobial
susceptibility
testing
(
Kirby
-
Bauer
,
MIC
)
✓
Anaerobic
culture
and
incubation
systems
✓
Fungal
and
mycobacterial
culture
✓
Culture
media
preparation
and
sterility
checks
✓
Nucleic
acid
extraction
(
manual
and
automated
platforms
)
✓
Real
-
time
PCR
and
RT
-
PCR
✓
Nucleic
acid
amplification
testing
(
NAAT
)
✓
Sanger
sequencing
and
NGS
library
preparation
✓
Viral
load
quantification
(
HIV
-1,
HCV
,
HBV
)
✓
Genotyping
and
mutation
analysis
✓
Gel
electrophoresis
and
capillary
electrophoresis
✓
Contamination
control
and
unidirectional
workflow
✓
Molecular
assay
validation
and
limit
of
detection
studies
✓
Immunofluorescence
assays
(
ANA
,
ANCA
)
✓
Infectious
disease
serology
(
HIV
,
syphilis
,
hepatitis
)
✓
Rheumatoid
factor
and
complement
assays
✓
Flow
cytometry
immunophenotyping
✓
HLA
typing
and
crossmatching
✓
Immunodiffusion
and
immunoelectrophoresis
✓
Antibody
titration
and
serial
dilution
technique
✓
Cold
agglutinin
and
direct
antiglobulin
(
DAT
)
testing
✓
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07
Quality
Assurance
&
Regulatory
08
Data
,
Documentation
&
Instrumentation
CLIA
,
CAP
,
and
ISO
15189
compliance
✓
Internal
quality
control
(
Levey
-
Jennings
,
Westgard
rules
)
✓
Proficiency
testing
(
PT
)
and
external
quality
assessment
✓
Method
validation
and
verification
:
accuracy
,
precision
,
linearity
✓
Reference
interval
establishment
and
verification
✓
Root
cause
analysis
and
corrective
/
preventive
action
(
CAPA
)
✓
Document
control
and
SOP
authoring
✓
Instrument
calibration
and
preventive
maintenance
✓
Biosafety
and
chemical
hygiene
plan
adherence
✓
Laboratory
Information
System
(
LIS
)
and
LIMS
operation
✓
Electronic
medical
record
(
EMR
)
result
reporting
✓
Data
entry
,
result
verification
,
and
delta
checks
✓
Critical
value
reporting
and
documentation
✓
Statistical
analysis
(
Excel
,
R
,
SPSS
)
✓
Instrument
troubleshooting
and
error
resolution
✓
Barcode
scanning
and
automation
line
management
✓
Inventory
and
reagent
lot
management
✓
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